different strains, that is, slight genetically different. It is also likely that if antibiotic sensitivity tests were run on these isolates, the results would vary against the different antibiotics used. The Kirby- test for antibiotic susceptibility, called the disc diffusion test, is a standard that has been used for years. First
ANTIBIOTIC SENSITIVITY TESTING I. OBJECTIVES • To utilize specific monitoring techniques to evaluate the susceptibility of a microbe to different antibiotics. • To distinguish the range of activity of an antibiotic. • To recognize and define advantages and limitations of two different susceptibility testing procedures. II. INTRODUCTION
MBK – Lab Report Name: _____ Section: _____ Module 11, Experiment 10: Antibiotic Sensitivity The Kirby- Test Interpret your results as described below. INTERPRETATION: • Place the metric ruler across the zone of inhibition, at the widest diameter, and …
experience working in a clinical microbiology laboratory will ﬁnd the information in this manual to have direct application for their susceptibility testing methods. Labo-ratory directors should ﬁnd this information useful as they design new procedures and update policies for susceptibility testing. This manual also could be a resource for
KIRBY- TEST for Antibiotic Susceptibility The Kirby- test for antibiotic susceptibility, called the disc diffusion test, is a standard that has been used for years. It has been superceded in clinical labs by automated tests. But the K-B is still used in some some labs, or used with certain bacteria that automation does not work well with.
Antibiotic Sensitivity Test. Work in a group of four. One pair of the group of four use the five TSA blood plates: Label the bottom of four of your TSA-blood agar plates with your name, date, and the name of the bacteria that you'll be testing for antibiotic sensitivity. Save the 5th plate for the unknown.
The Kirby test is a qualitative assay whereby disks of filter paper are impregnated with a single concentration of different antibiotics or any chemicals that will diffuse from the disk into the agar. The selected antibiotic disks are placed on the surface of an agar plate which has already been inoculated with test bacteria.
Antibiotic Susceptibility Testing by the CDS Method Chapter 1. Introduction Eighth Edition 8 1. Introduction The first published description of the CDS Test appeared in "Pathology" in 19751. Diagnostic laboratories in Australasia soon adopted the CDSand it became the most commonly used method of susceptibility testing in this country.
Purposes of antimicrobial susceptibility testing. . Low equipment cost, Easy to change antibiotics tested, Easy to see test failures, back-up when instruments fail Disadvantages: Not approved for all types of bacteria, manual test--labor cost, chance for technical mistakes, requires 16-24 h, No MIC result, reqs refrigerator & incubator space .
The CDS test is a high potency disc diffusion method of antibiotic susceptibility testing where the results are calibrated to an internationally accepted quantitative technique. It was developed in the late 1960s and made available to laboratories in Australia in an effort to improve their performance in susceptibility testing.